Nikon Widefield - Specifications
General Information
Microscope type: Widefield
Purpose: Rapid and long-term 2D imaging
FPbase link: https://www.fpbase.org/microscope/hkGFJUe7zVWYEuD4ev85ie/
Equipment information
Location: JSCBB C350C
- HHMI 301492
- HHMI 311898
- UCB 217448
Sample types
|
Fixed Cells |
Yes |
| Live Cells | Yes |
Software
- Nikon 5.20.02
Environmental control
- CO2, Humidity, Temperature
Light source
- Excelitas Xylis LED
Filters
| Excitation | Emission | Fluors |
||||||
| Center | Width | Min | Max | Center | Width | Min | Max | |
| 350 | 50 | 325 | 375 | 460 | 50 | 435 | 485 | DAPI, BFP |
| 470 | 40 | 450 | 490 | 525 | 50 | 500 | 550 | GFP, FITC, Alexa Fluor 488 |
| 560 | 40 | 540 | 580 | 630 | 75 | 592.5 | 667.5 | TRITC, Texas Red, dsRed |
| 620 | 60 | 590 | 650 | 700 | 75 | 662.5 | 737.5 | Cy5, Alexa Fluor 647, Draq5 |
Objectives
| Magnification | NA | Immersion medium |
| 4 | 0.2 | Air |
| 10 | 0.45 | Air |
| 20 | 0.75 | Air |
| 40 | 0.95 | Air |
| 100 | 1.4 | Oil |
Detectors
- Hamamatsu Flash 4.0 v3 (sCMOS camera)
- 16-bit detector: pixel intensity values from 0 - 65,535
- Nikon DS-Fi3 (RGB color camera)
- 8-bit detector: pixel intensity values from 0 - 255
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