Nikon Widefield Specifications
Microscope type: Widefield
Purpose: Rapid and long-term 2D imaging
FPbase link: https://www.fpbase.org/microscope/hkGFJUe7zVWYEuD4ev85ie/
Location: JSCBB C305D
Equipment tags:
Sample types
| Software |
NIS Elements v5.20.02 |
| HASP |
26FCA24 |
| Base |
Nikon TiE |
| XY Stage |
Motorized |
| Z |
Ti Z drive |
| Autofocus |
PFS 3 |
| Environment Control |
Oko Labs enclosure: CO2, Humidity, Temperature |
| Light Source |
Excelitas Xylis LED |
Filters
| Excitation |
Emission |
|
|
| Center |
Width |
Min |
Max |
Center |
Width |
Min |
Max |
Fluorophores |
Vendor |
| 350 |
50 |
325 |
375 |
460 |
50 |
435 |
485 |
DAPI, BFP |
Chroma |
| 470 |
40 |
450 |
490 |
525 |
50 |
500 |
550 |
GFP, FITC, Alexa Fluor 488 |
Chroma |
| 560 |
40 |
540 |
580 |
630 |
75 |
592.5 |
667.5 |
TRITC, Texas Red, dsRed |
Dichroic T585lpxr |
| 620 |
60 |
590 |
650 |
700 |
75 |
662.5 |
737.5 |
Cy5, Alexa Fluor 647, Draq5 |
Chroma |
Objectives
| Magnification |
NA |
Immersion medium |
| 4 |
0.2 |
Air |
| 10 |
0.45 |
Air |
| 20 |
0.75 |
Air |
| 40 |
0.95 |
Air |
| 100 |
1.4 |
Oil |
Detectors
| sCMOS |
| Hamamatsu Flash 4.0 v3 |
| S/N: 170216-SYS-006488 |
No Comments