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Nikon Widefield - Specifications

General Information

Microscope type: Widefield
Purpose: Rapid and long-term 2D imaging
FPbase link: https://www.fpbase.org/microscope/hkGFJUe7zVWYEuD4ev85ie/

Equipment information

Location: JSCBB C350C

CU Tags: 

  • HHMI 301492
  • HHMI 311898
  • UCB 217448

Sample types

Fixed Cells

 Yes
Live Cells Yes

Software

  • Nikon 6.10.02

Base

  • Nikon Ti-2

Environmental control

  • CO2, Humidity, Temperature

Light source

  • Excelitas Xylis LED

Filters

Excitation Emission Fluors
Center Width Min Max Center Width Min Max
350 50 325 375 460 50 435 485 DAPI, BFP
470 40 450 490 525 50 500 550 GFP, FITC, Alexa Fluor 488
560 40 540 580 630 75 592.5 667.5 TRITC, Texas Red, dsRed
620 60 590 650 700 75 662.5 737.5 Cy5, Alexa Fluor 647, Draq5

Objectives

Magnification NA Immersion medium
4 0.2 Air
10 0.45 Air
20 0.75 Air
40 0.95 Air
100 1.4 Oil

Detectors

  • Hamamatsu Orca-Fusion (sCMOS camera)
    • 2304 x 2304 pixels 
    • 16-bit detector: pixel intensity values from 0 - 65,535
  • Nikon DS-Fi3 (RGB color camera)
    • 8-bit detector: pixel intensity values from 0 - 255

History

Date Changes
03-04-2026

Nikon Ti2 microscope base upgrade: filters and objectives unchanged.
Camera switched from Hamamatsu Orca-Flash4.0 to Hamamatsu Orca-Fusion.

No DIC components after base upgrade.

 

See operational changes here.
11-21-2025

Attempt Nikon Ti2 microscope base upgrade (controller issue).

Old widefield put back together.
Camera changed from Hamamatsu Orca-Flash4.0 to Hamamatsu Orca-Fusion.
01-13-2025

Stage replaced (Y encoder nonfunctional)

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