Nikon Widefield
Basic use
This protocol covers the basics of mounting your sample on the microscope and setting up the perf...
Moving the sample
The microscope contains a motorized stage which moves the sample. The stage is controlled using t...
Using the Perfect Focus System (PFS)
The perfect focus system locates the coverslip and continually adjusts the objective height to fo...
Using Live View
In live view, the microscope will continuously acquire images, allowing you to preview settings a...
Changing the optical configuration
The following sections describe the different settings that can be changed for each channel. Thes...
Adjusting the lookup tables
When acquiring images, the camera records the intensity of light falling on each pixel. This resu...
Acquiring a single image
This protocol explains how to take a simple image with just a single frame and a single channel. ...
Using the ND acquisition panel
This page describes how to use the ND acquisition panel to set up more complex imaging, including...
Cleaning up
It is important that you clean up after yourself after using the microscope, as well as transferr...
Nikon Widefield Specifications
General Information Microscope type: WidefieldPurpose: Rapid and long-term 2D imagingFPbase link...
Online learning resources by Nikon
Nikon is now offering learning resources available online. To request a login password, please em...