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Bruker TruLive3D

Training manual for the Bruker TruLive3D Light Sheet Microscope

Bruker TruLive3D training protocol

Training Program  Bruker TruLive3D Light Sheet Microscope    Session 1 – Instrument and Operat...

Bruker TruLive3D specifications and primary uses

Bruker TruLive3D main specifications The TruLive3D has 4 imaging channels at 405nm, 488nm, 561...

1. Bruker TruLive3D start-up procedure

  a.      Turn on the microscope In case of emergency, push the big red button: it turns off ...

2. Bruker TruLive3D microscope overview

a.   The Bruker TruLive3D is a dual sided illumination light sheet microscope. The dual light she...

3. Bruker TruLive3D Lux-Bundle overview

Once the microscope, cameras and temperature controller and gas mixer are on it is time to op...

4. Bruker Trulive3D calibration

Calibration of the laser beams is critical for optimal imaging and should be done prior to ever...

5. Bruker TruLive3D Finding the sample

Make sure you performed the calibration of the system before lowering your sample in the chambe...

6. Bruker TruLive3D Experiment

This page will guide through the steps to set-up a basic experiment. 1. Setting up an experime...

7. Bruker TruLive3D Data management

Data acquired on the Bruker TrLive3D will typically be very large, on the order of TeraBytes of...

8. Bruker TruLive3D Using the photo-manipulation (PM) feature

The Bruker TruLive3D has a photo-manipulation (PM) feature that you can use to ablate specific ...

9. Bruker TruLive3D shut-down procedure

Once you are done with your experiment, please proceed with the following shut-down procedure: ...

Bruker TruLive3D Sample preparation guidelines

This page contains some tips for sample preparation for the Bruker Trulive 3D. It is not exhausti...

Bruker TruLive3D emission filters and fluorophores spectrum

This page will help you select which filter combination you need for most commonly used fluorop...

Bruker TruLive3D Data management pipeline

Bruker TruLive3D Data visualization in Fiji

The data generated by the Bruker TruLive3D contains a Fiji header named bdv.h5.  Fiji is an open...