Microscope manuals
How to use our microscopes
Nikon Widefield
Basic use
This protocol covers the basics of mounting your sample on the microscope and setting up the perf...
Moving the sample
The microscope contains a motorized stage which moves the sample. The stage is controlled using t...
Using the Perfect Focus System (PFS)
The perfect focus system locates the coverslip and continually adjusts the objective height to fo...
Using Live View
In live view, the microscope will continuously acquire images, allowing you to preview settings a...
Changing the optical configuration
The following sections describe the different settings that can be changed for each channel. Thes...
Adjusting the lookup tables
When acquiring images, the camera records the intensity of light falling on each pixel. This resu...
Acquiring a single image
This protocol explains how to take a simple image with just a single frame and a single channel. ...
Using the ND acquisition panel
This page describes how to use the ND acquisition panel to set up more complex imaging, including...
Cleaning up
It is important that you clean up after yourself after using the microscope, as well as transferr...
Nikon Widefield Specifications
General Information Microscope type: WidefieldPurpose: Rapid and long-term 2D imagingFPbase link...
Online learning resources by Nikon
Nikon is now offering learning resources available online. To request a login password, please em...
Nikon Spinning Disc Confocal
Nikon SDC - Basic set-up
This protocol covers the basics of turning on the microscope, mounting your sample, and acquiring...
Nikon SDC - Mode of operation
Nikon SDC microscope features This microscope offers several capabilities, it has two light so...
Nikon SDC - Shut-down and clean up
When you are done imaging, remove your sample from the microscope and place it on a kimwipe or ot...
Nikon SDC - Specifications
Type: Spinning disc confocalPurpose: Rapid 2D and 3D imaging of samplesFixed cells: YesLive cells...
Using the Perfect Focus System (PFS)
The perfect focus system locates the coverslip and continually adjusts the objective height to fo...
Online learning resources by Nikon
Nikon is now offering learning resources available online. To request a login password, please em...
Tutorial Videos
Nikon A1R Laser Scanning Confocal
Nikon A1R - Overview
Basics of laser scanning confocal A laser scanning confocal microscope produces high resolution ...
Nikon A1R - Basic setup
This guide describes how to turn on the microscope and mount your sample on the microscope. If...
Nikon A1R - Moving the sample
The microscope contains a motorized stage which moves the sample. The stage is controlled using t...
Nikon A1R - Mode of operation
Overview of the OC (Optical Configuration) control panel The OC Panel contains the different li...
Shut Down and clean up procedures
When you are done imaging, remove your sample from the microscope and place it on a kimwipe or ot...
Using the Perfect Focus System (PFS)
The perfect focus system locates the coverslip and continually adjusts the objective height to fo...
Online learning resources by Nikon
Nikon is now offering learning resources available online. To request a login password, please em...
Nikon NSTORM
Nikon NSTORM - Basic Set-up
Getting the system ready If the microscope is off, turn on the microscope following the prot...
Nikon NSTORM - Mode of operation
Microscope Controls To control the microscope stage, you will use the joystick. The central knob...
Nikon NSTORM - Shut-down and clean up
When you are done imaging, remove your sample from the microscope and place it on a kimwipe or ot...
Nikon NSTORM Specifications
Using the Perfect Focus System (PFS)
The perfect focus system locates the coverslip and continually adjusts the objective height to fo...
Online learning resources by Nikon
Nikon is now offering learning resources available online. To request a login password, please em...
PerkinElmer Opera Phenix
PerkinElmer Opera Phenix Specifications
General information Microscope type: Spinning disk confocal and widefieldPurpose: High-throughpu...
Exporting a video file
This document explains how to export a video file from a time-lapse dataset. Click on a well i...
Exporting and deleting files
After your session, you should backup your data from the Opera Phenix. There are two main file fo...
PerkinElmer - Opera Phenix manual
This microscope has excellent online resources that you can consult after completing your in-pers...
Molecular Devices ImageXpress
Workstations
Bruker TruLive3D
Training manual for the Bruker TruLive3D Light Sheet Microscope
Bruker TruLive3D training protocol
Training Program Bruker TruLive3D Light Sheet Microscope Session 1 – Instrument and Operat...
1. Bruker TruLive3D start-up procedure
a. Turn on the microscope In case of emergency, push the big red button: it turns off the...
2. Bruker TruLive3D microscope overview
a. The Bruker TruLive3D is a dual sided illumination light sheet microscope. The dual light she...
3. Bruker TruLive3D Lux-Bundle overview
Once the microscope, cameras and temperature controller and gas mixer are on it is time to open...
4. Bruker Trulive3D calibration
Calibration of the laser beams is critical for optimal imaging and should be done prior to every ...
5. Bruker TruLive3D Finding the sample
Make sure you performed the calibration of the system before lowering your sample in the chamber....
6. Bruker TruLive3D Experiment
This page will guide through the steps to set-up a basic experiment. 1. Setting up an experiment...
7. Bruker TruLive3D Data management
Data acquired on the Bruker TrLive3D will typically be very large, on the order of TeraBytes of d...
8. Bruker TruLive3D Using the photo-manipulation (PM) feature
The Bruker TruLive3D has a photo-manipulation (PM) feature that you can use to ablate specific ar...
9. Bruker TruLive3D shut-down procedure
Once you are done with your experiment, please proceed with the following shut-down procedure: ...
Bruker TruLive3D emission filters and fluorophores spectrum
This page will help you select which filter combination you need for most commonly used fluoropho...
Bruker TruLive3D Data management pipeline
Microscopy Essentials
Essentials about cameras, filter cubes and more