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PerkinElmer Opera Phenix manual

Please, see the following PDFs for the Opera Phenix manual:

Manual to adjust imaging settings and take images
Manual to use internal analysis software
Manual to use the automation software


Comparisons of objectives and imaging modes:

Phenix_MagnificationComparison-min.jpg

 

Phenix_Con-v-nonCon-min.jpg

 

Phenix_Seq-v-Sim-min.jpg

 

Phenix_binning-min.jpg

 

Comparison of drift of fluorescent beads by timelapse type:

Timelapse with the plate in the Phenix the whole time:

merged.gif

 

Timelapse with the plate removed and put back using the automation/robot:

BeadsOverTime_withAutomation.gif

* Regions that get dimmer were caused by condensation on the bottom of the plate that occurs as the robot arm moves the plate from the incubator to the Phenix and back.