# Nikon Widefield Specifications

### General Information

**Microscope type:** Widefield  
**Purpose:** Rapid and long-term 2D imaging  
**FPbase link:** [https://www.fpbase.org/microscope/hkGFJUe7zVWYEuD4ev85ie/](https://www.fpbase.org/microscope/hkGFJUe7zVWYEuD4ev85ie/)

### Equipment information

**Location:** JSCBB C305D

**Equipment tags:**

- HHMI 301492
- HHMI 311898

### Sample types

- Fixed cells
- Live cells

### Equipment information

<table id="bkmrk-software-nis-element" style="border-collapse: collapse; width: 550px;" width="181"><colgroup><col style="width: 122px;" width="117"></col><col style="width: 427px;" width="64"></col></colgroup><tbody><tr style="height: 15.0pt;"><td height="20" style="width: 88pt; height: 15.0pt;" width="117">Software</td><td style="width: 48pt;" width="64">NIS Elements v5.20.02</td></tr><tr style="height: 15.0pt;"><td height="20" style="height: 15.0pt;">HASP</td><td>26FCA24</td></tr><tr style="height: 15.0pt;"><td height="20" style="height: 15.0pt;">Base</td><td>Nikon TiE</td></tr><tr style="height: 15.0pt;"><td height="20" style="height: 15.0pt;">XY Stage</td><td>Motorized</td></tr><tr style="height: 15.0pt;"><td height="20" style="height: 15.0pt;">Z</td><td>Ti Z drive</td></tr><tr style="height: 15.0pt;"><td height="20" style="height: 15.0pt;">Autofocus</td><td>PFS 3</td></tr><tr><td height="20" style="width: 88pt; height: 15.0pt;" width="117">Environment Control</td><td style="width: 48pt;" width="64">Oko Labs enclosure: CO2, Humidity, Temperature</td></tr><tr><td height="20" style="height: 15.0pt;">Light Source</td><td>Excelitas Xylis LED</td></tr></tbody></table>

### Filters

<table border="1" id="bkmrk-excitation-emission-" style="border-collapse: collapse; width: 817px; height: 402px; border-color: rgb(35, 111, 161); border-style: solid;" width="960"><colgroup><col span="10" style="width: 82px;" width="64"></col></colgroup><tbody><tr style="height: 15.0pt;"><th class="xl20 align-center" colspan="4" height="20" style="width: 192pt; height: 15pt; border-color: rgb(35, 111, 161);" width="256">Excitation</th><th class="xl20 align-center" colspan="4" height="20" style="width: 192pt; height: 15pt; border-color: rgb(35, 111, 161);" width="256">Emission</th><th style="width: 48pt; border-color: rgb(35, 111, 161);" width="64"> </th><th style="width: 48pt; border-color: rgb(35, 111, 161);" width="64"> </th></tr><tr style="height: 15.0pt;"><th class="align-center" height="20" style="height: 15pt; border-color: rgb(35, 111, 161);">Center</th><th class="align-center" style="border-color: rgb(35, 111, 161);">Width</th><th class="align-center" style="border-color: rgb(35, 111, 161);">Min</th><th class="align-center" style="border-color: rgb(35, 111, 161);">Max</th><th class="align-center" style="border-color: rgb(35, 111, 161);">Center</th><th class="align-center" style="border-color: rgb(35, 111, 161);">Width</th><th class="align-center" style="border-color: rgb(35, 111, 161);">Min</th><th class="align-center" style="border-color: rgb(35, 111, 161);">Max</th><th class="align-center" style="border-color: rgb(35, 111, 161);">Fluorophores</th><th class="align-center" style="border-color: rgb(35, 111, 161);">Vendor</th></tr><tr style="height: 15.0pt;"><td align="right" class="align-center" height="20" style="height: 15pt; border-color: rgb(35, 111, 161);">350</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">50</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">325</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">375</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">460</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">50</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">435</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">485</td><td style="border-color: rgb(35, 111, 161);">DAPI, BFP</td><td style="border-color: rgb(35, 111, 161);">Chroma</td></tr><tr style="height: 15.0pt;"><td align="right" class="align-center" height="20" style="height: 15pt; border-color: rgb(35, 111, 161);">470</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">40</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">450</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">490</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">525</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">50</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">500</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">550</td><td style="border-color: rgb(35, 111, 161);">GFP, FITC, Alexa Fluor 488</td><td style="border-color: rgb(35, 111, 161);">Chroma</td></tr><tr style="height: 15.0pt;"><td align="right" class="align-center" height="20" style="height: 15pt; border-color: rgb(35, 111, 161);">560</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">40</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">540</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">580</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">630</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">75</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">592.5</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">667.5</td><td style="border-color: rgb(35, 111, 161);">TRITC, Texas Red, dsRed</td><td style="border-color: rgb(35, 111, 161);">Dichroic T585lpxr</td></tr><tr style="height: 15.0pt;"><td align="right" class="align-center" height="20" style="height: 15pt; border-color: rgb(35, 111, 161);">620</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">60</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">590</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">650</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">700</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">75</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">662.5</td><td align="right" class="align-center" style="border-color: rgb(35, 111, 161);">737.5</td><td style="border-color: rgb(35, 111, 161);">Cy5, Alexa Fluor 647, Draq5</td><td style="border-color: rgb(35, 111, 161);">Chroma</td></tr></tbody></table>

### Objectives

<table border="1" id="bkmrk-model-magnification-" style="border-collapse: collapse; border: 1px solid rgb(52, 73, 94); width: 581px;" width="192"><colgroup><col span="3" style="width: 193px;" width="64"></col></colgroup><tbody><tr style="height: 15.0pt;"><td class="align-center" height="20" style="width: 24pt; height: 15pt; border-color: rgb(52, 73, 94); border-width: 1px;" width="64">**Magnification**</td><td class="align-center" style="width: 48pt; border-color: rgb(52, 73, 94); border-width: 1px;" width="64">**NA**</td><td class="align-center" style="width: 48pt; border-color: rgb(52, 73, 94); border-width: 1px;" width="64">**Immersion medium**</td></tr><tr style="height: 15.0pt;"><td align="right" class="align-center" height="20" style="height: 15pt; border-color: rgb(52, 73, 94); border-width: 1px;">4</td><td align="right" class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">0.2</td><td class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">Air</td></tr><tr style="height: 15.0pt;"><td align="right" class="align-center" height="20" style="height: 15pt; border-color: rgb(52, 73, 94); border-width: 1px;">10</td><td align="right" class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">0.45</td><td class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">Air</td></tr><tr style="height: 15.0pt;"><td align="right" class="align-center" height="20" style="height: 15pt; border-color: rgb(52, 73, 94); border-width: 1px;">20</td><td align="right" class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">0.75</td><td class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">Air</td></tr><tr style="height: 15.0pt;"><td align="right" class="align-center" height="20" style="height: 15pt; border-color: rgb(52, 73, 94); border-width: 1px;">40</td><td align="right" class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">0.95</td><td class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">Air</td></tr><tr style="height: 15.0pt;"><td align="right" class="align-center" height="20" style="height: 15pt; border-color: rgb(52, 73, 94); border-width: 1px;">100</td><td align="right" class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">1.4</td><td class="align-center" style="border-color: rgb(52, 73, 94); border-width: 1px;">Oil</td></tr></tbody></table>

### Detectors

<table id="bkmrk-scmos-hamamatsu-flas" style="border-collapse: collapse; width: 187px;" width="64"><colgroup><col style="width: 186px;" width="64"></col></colgroup><tbody><tr style="height: 15.0pt;"><td height="20" style="width: 48pt; height: 15.0pt;" width="64">sCMOS</td></tr><tr style="height: 15.0pt;"><td height="20" style="height: 15.0pt;">Hamamatsu Flash 4.0 v3</td></tr><tr style="height: 15.0pt;"><td height="20" style="height: 15.0pt;">S/N: 170216-SYS-006488</td></tr></tbody></table>