Nikon SDC - Specifications

Type: Spinning disc confocal
Purpose: Rapid 2D and 3D imaging of samples
Fixed cells: Yes
Live cells: Yes
FPBase link: https://www.fpbase.org/microscope/X6ne45wr4DYFPsgc5nPrKo
Tag: None
Room: C350A

Software:

• NIS Elements - v5.42.03

HASP: 293CDCAA
Base: Nikon TiE
XY Stage: Motorized

Z:

• Ti Z drive
• MCL Piezo Z - 100 µm

Autofocus: PFS 3

Environment control: 

• Oko Labs enclosure: CO2, Humidity, Temperature

Scan head: Yokogawa CSU-X1

Light source:

• LUNV22 70186
• Lasers: 405, 445, 488, 515, 561, 594, 640

Emission Filters

Vendor	Model	Center	Width	Min	Max	Common Fluorophores
Chroma	ETDAPI-Cy5 m Lot 314725	437	18	428	446	DAPI. BFP
Chroma	ET482/27m	482	34	465	499	CFP
Chroma	ET525/50m	525	50	500	550	GFP, FITC, Alexa Fluor 488
Chroma	ET540/30m	540	30	525	555	YFP
Chroma	ET620/60m	620	60	590	650	TRITC, Texas Red, dsRed, Alexa Fluor 568
Chroma	ET630/30m	630	30	615	645	mCherry
Chroma	ETDAPI-Cy5 m Lot 314725	705	68	671	739	Cy5, Alexa Fluor 647, Draq5

Dichroics

• Chroma ZT405/488/561/640rpcv2
• Chroma ZT44/514/594rpc

Objectives

Magnification	NA	Medium
4	0.2	Air
10	0.45	Air
20	0.75	Air
40	0.95	Air
100	1.4	Oil

Detectors

• EMCCD
• Andor IXon 888 Ultra
• S/N: X-9963