Nikon SDC - Specifications
General Information
Microscope type: Spinning disk confocal
Purpose: Rapid 2D and 3D imaging
FPbase link: https://www.fpbase.org/microscope/qcBX84Msxp9cBTjLn6GoQD/
Equipment information
Location: JSCBB C350A
CU Tag: UCB 211567
Sample types
|
Fixed Cells |
Yes |
| Live Cells | Yes |
Software
- Nikon 5.42.03
Base
- Nikon Ti-E
Environmental control
- CO2, Humidity, Temperature
Light source
- LUNV22
Laser lines
- 405 nm
- 445 nm
- 488 nm
- 515 nm
- 561 nm
- 594 nm
- 640 nm
Filters
| Emission | Example fluorophores |
|||
| Center (nm) | Width (nm) | Min (nm) | Max (nm) | |
| 437 | 18 | 428 | 446 | DAPI, BFP |
| 482 | 34 | 465 | 499 | CFP |
| 525 | 50 | 500 | 550 | GFP, FITC, Alexa Fluor 488 |
| 540 | 30 | 525 | 555 | YFP |
| 620 | 60 | 590 | 650 | TRITC, Texas Red, dsRed |
| 630 | 30 | 615 | 645 | mCherry |
| 705 | 68 | 671 | 739 | Cy5, Alexa Fluor 647, Draq5 |
Objectives
| Magnification | NA | Immersion medium |
| 4 | 0.2 | Air |
| 10 | 0.45 | Air |
| 20 | 0.75 | Air |
| 40 | 0.95 | Air |
| 100 | 1.4 | Oil |
Z-drives
- Ti Z drive
- MCL Piezo Z - 100 µm
Scan head
- Yokogawa CSU-X1
Detectors
- Andor iXon 888 ultra (EMCCD camera)
- 16-bit detector: pixel intensity values from 0 - 65,535
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