Nikon Widefield Specifications
General Information
Microscope type: Widefield
Purpose: Rapid and long-term 2D imaging
FPbase link: https://www.fpbase.org/microscope/hkGFJUe7zVWYEuD4ev85ie/
Equipment information
Location: JSCBB C305D
- HHMI 301492
- HHMI 311898
Sample types
- Fixed cells
- Live cells
Equipment information
| Software | NIS Elements v5.20.02 |
| HASP | 26FCA24 |
| Base | Nikon TiE |
| XY Stage | Motorized |
| Z | Ti Z drive |
| Autofocus | PFS 3 |
| Environment Control | Oko Labs enclosure: CO2, Humidity, Temperature |
| Light Source | Excelitas Xylis LED |
Filters
| Excitation | Emission | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Center | Width | Min | Max | Center | Width | Min | Max | Fluorophores | Vendor |
| 350 | 50 | 325 | 375 | 460 | 50 | 435 | 485 | DAPI, BFP | Chroma |
| 470 | 40 | 450 | 490 | 525 | 50 | 500 | 550 | GFP, FITC, Alexa Fluor 488 | Chroma |
| 560 | 40 | 540 | 580 | 630 | 75 | 592.5 | 667.5 | TRITC, Texas Red, dsRed | Dichroic T585lpxr |
| 620 | 60 | 590 | 650 | 700 | 75 | 662.5 | 737.5 | Cy5, Alexa Fluor 647, Draq5 | Chroma |
Objectives
| Magnification | NA | Immersion medium |
| 4 | 0.2 | Air |
| 10 | 0.45 | Air |
| 20 | 0.75 | Air |
| 40 | 0.95 | Air |
| 100 | 1.4 | Oil |
Detectors
| sCMOS |
| Hamamatsu Flash 4.0 v3 |
| S/N: 170216-SYS-006488 |