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This protocol covers the basics of mounting your sample on the microscope and setting up the perfect focus.  If the microscope is off, turn on the microscope following the protocol taped to the wall. Start Nikon Elements: Click on the icon on the deskto...

The perfect focus system locates the coverslip and continually adjusts the objective height to follow any drift in the system. Engaging the PFS Only if using the 100x oil objective, first raise the objective by turning the z-axis control until the oil cont...

The microscope contains a motorized stage which moves the sample. The stage is controlled using the joystick. The sections below describe how to move the sample in XY and Z directions. XY position The XY position of the stage can be controlled by using the j...

In live view, the microscope will continuously acquire images, allowing you to preview settings and focus the sample.  These are general instructions to use Live View mode:  Make sure an optical configuration is selected by clicking on the appropriate butt...

The following sections describe the different settings that can be changed for each channel. These parameters are saved independently for each channel. Changing channels Select the desired optical configuration by clicking on the button in the OC Panel. Th...

When acquiring images, the camera records the intensity of light falling on each pixel. This results in a greyscale image that is later colored by the Nikon Elements software. The apparent brightness of each pixel can be adjusted using a "lookup table" (LUT). ...