Bruker TruLive3D emission filters and fluorophores spectrum

This page will help you select which filter combination you need for most commonly used fluorophores on the Bruker TruLive3D.

The Bruker TruLive3D light sheet microscope has two imaging cameras with two motorized filter wheels and two sets of filters. It is designed to split different emission wavelengths between the two cameras for simultaneous imaging with two different emission channels.

The "Long Camera" will record the fluorescence emitting at the longest wavelength and the "Short Camera" will record the fluorescence emitting at the shortest wavelength.

When you set up your experiment you want to make sure that you select the best filter option for each camera so that the fluorescence from one fluorophore does not mix up with the other.

The Bruker TruLive3D has the following emission filters options:

Filter wheel Short Camera: BP418-462    LP498    BP497-554    BP580-627   BP610-651

Filter wheel Long Camera:                         LP498    BP497-554    BP580-627    BP610-651    BP655-704

Below is a list of the most commonly used fluorophores and the filters you should use:

Fluorophores	Filter	Bruker Laser line
GFP	BP 497-554	488
DAPI	BP 418-462	405
TRITC	BP 580-627	561
mCherry	BP 580-627	561
AlexaFluor488	BP 497-554	488
AlexaFluor555	BP 580-627	561
AlexaFluor594	BP 580-627 or BP 610-651	561
AlexaFluor647	BP 655-704	640

To select the appropriate filters you need to know the emission peak wavelength of the fluorophores you are using.

For a better understanding on how to select the correct filter, you can look at the excitation/emission spectrums from fpbase.org   or aatbio.com for example. When you click on the link you can use the interactive spectrum viewer and see what the filters cover.

GFP : https://www.fpbase.org/protein/egfp/

mCherry : https://www.fpbase.org/protein/mcherry/ 

DAPI : https://www.aatbio.com/fluorescence-excitation-emission-spectrum-graph-viewer/dapi_4_6_diamidino_2_phenylindole

TRITC : https://www.aatbio.com/fluorescence-excitation-emission-spectrum-graph-viewer/tritc_tetramethylrhodamine_isothiocyanate

AlexaFluor488 : https://www.aatbio.com/fluorescence-excitation-emission-spectrum-graph-viewer/alexa_fluor_488

AlexaFluor555 : https://www.aatbio.com/fluorescence-excitation-emission-spectrum-graph-viewer/xfd555_alexa_fluor_555_equivalent

AlexaFluor594 : https://www.aatbio.com/fluorescence-excitation-emission-spectrum-graph-viewer/xfd594_alexa_fluor_594_equivalent

AlexaFluor647 : https://www.aatbio.com/fluorescence-excitation-emission-spectrum-graph-viewer/xfd647_alexa_fluor_647_equivalent

If your fluorophores are not on this page please let us know and we will update this page for you!