# Nikon SDC - Specifications

Type: Spinning disc confocal  
Purpose: Rapid 2D and 3D imaging of samples  
Fixed cells: Yes  
Live cells: Yes  
FPBase link: [https://www.fpbase.org/microscope/X6ne45wr4DYFPsgc5nPrKo](https://www.fpbase.org/microscope/X6ne45wr4DYFPsgc5nPrKo)  
Tag: None  
Room: C350A

Software:

- NIS Elements - v5.42.03

HASP: 293CDCAA  
Base: Nikon TiE  
XY Stage: Motorized

Z:

- Ti Z drive
- MCL Piezo Z - 100 µm

Autofocus: PFS 3

Environment control:

- Oko Labs enclosure: CO2, Humidity, Temperature

Scan head: Yokogawa CSU-X1

Light source:

- LUNV22 70186
- Lasers: 405, 445, 488, 515, 561, 594, 640

Emission Filters

<table border="1" id="bkmrk-vendor-model-center-"><colgroup><col></col><col></col><col></col><col></col><col></col><col></col><col></col></colgroup><tbody><tr><td>Vendor</td><td>Model</td><td>Center</td><td>Width</td><td>Min</td><td>Max </td><td>Common Fluorophores</td></tr><tr><td height="20" width="64">Chroma</td><td width="64">ETDAPI-Cy5 m Lot 314725</td><td align="right" height="20" width="64">437</td><td align="right" width="64">18</td><td align="right" width="64">428</td><td align="right" width="64">446</td><td width="64">DAPI. BFP</td></tr><tr><td height="20">Chroma</td><td>ET482/27m</td><td align="right" height="20">482</td><td align="right">34</td><td align="right">465</td><td align="right">499</td><td>CFP</td></tr><tr><td height="20">Chroma</td><td>ET525/50m</td><td align="right" height="20">525</td><td align="right">50</td><td align="right">500</td><td align="right">550</td><td>GFP, FITC, Alexa Fluor 488</td></tr><tr><td height="20">Chroma</td><td>ET540/30m</td><td align="right" height="20">540</td><td align="right">30</td><td align="right">525</td><td align="right">555</td><td>YFP</td></tr><tr><td height="20">Chroma</td><td>ET620/60m</td><td align="right" height="20">620</td><td align="right">60</td><td align="right">590</td><td align="right">650</td><td>TRITC, Texas Red, dsRed, Alexa Fluor 568</td></tr><tr><td height="20">Chroma</td><td>ET630/30m</td><td align="right" height="20">630</td><td align="right">30</td><td align="right">615</td><td align="right">645</td><td>mCherry</td></tr><tr><td height="20">Chroma</td><td>ETDAPI-Cy5 m Lot 314725</td><td align="right" height="20">705</td><td align="right">68</td><td align="right">671</td><td align="right">739</td><td>Cy5, Alexa Fluor 647, Draq5</td></tr></tbody></table>

Dichroics

- Chroma ZT405/488/561/640rpcv2
- Chroma ZT44/514/594rpc

Objectives

<table border="1" id="bkmrk-magnification-na-med"><colgroup><col></col><col></col><col></col></colgroup><tbody><tr><td height="20" width="64">Magnification</td><td width="64">NA</td><td width="64">Medium</td></tr><tr><td align="right" height="20">4</td><td align="right">0.2</td><td>Air</td></tr><tr><td align="right" height="20">10</td><td align="right">0.45</td><td>Air</td></tr><tr><td align="right" height="20">20</td><td align="right">0.75</td><td>Air</td></tr><tr><td align="right" height="20">40</td><td align="right">0.95</td><td>Air</td></tr><tr><td align="right" height="20">100</td><td align="right">1.4</td><td>Oil</td></tr></tbody></table>

Detectors

- EMCCD
- Andor IXon 888 Ultra
- S/N: X-9963