Nikon A1R - Specifications
General information
Microscope type: Laser scanning confocal
Purpose: 2D and 3D images of fixed and live samples
FPbase link: https://www.fpbase.org/microscope/YNE7RrmRWYDwRYVuCKxY2n/
Equipment information
Location: JSCBB C1B76
CU Tag: UCB 208134
Sample types
| Fixed Cells | Yes |
| Live Cells | Yes |
Software
- NIS Elements 5.42.03
Base
- Nikon Ti-E
Environmental control
- CO2, Humidity, Temperature
Laser lines
- 405 nm
- 488 nm
- 561 nm
- 640 nm
Filters
| Emission |
Example fluorophores |
|||
| Center (nm) | Width (nm) | Min (nm) | Max (nm) | |
| 450 | 50 | 425 | 475 | DAPI, BFP, CFP |
|
485 |
34 | 468 | 502 | CFP |
| 525 | 50 | 500 | 550 | GFP, FITC, Alexa 488 |
| 545 | 40 | 525 | 565 | YFP |
| 600 | 50 | 575 | 625 | TRITC, Texas Red, Alexa 568 |
| 685 | 70 | 650 | 720 | Cy5, Alexa 647, Draq5 |
Objectives
| Magnification | NA | Immersion medium |
| 4X | 0.20 | Air |
| 10X | 0.45 | Air |
| 20X | 0.75 | Air |
| 40X | 0.95 | Air |
| 100X | 1.4 | Oil |
Detectors
- Nikon PMT detectors for 405-nm and 488-nm laser lines
- GaAsp PMT detectors for 561-nm and 640-nm laser lines
- 12-bit detectors: pixel intensity values from 0 - 4095
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