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 For questions, comments or concerns please email us at biof-imaging@colorado.edu  This protocol covers the basics of turning on the microscope, mounting your sample, and acquiring a simple image. If the microscope is off, turn on the microscope (the proto...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  a.      Turn on the microscope In case of emergency, push the big red button: it turns off the entire system. When you turn it back on, turn it counter clockwise until it is...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  a.   The Bruker TruLive3D is a dual sided illumination light sheet microscope. The dual light sheet is projected on the sample by a pair of water immersion 10X 0.3NA microscope...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  This page will help you select which filter combination you need for most commonly used fluorophores on the Bruker TruLive3D. The Bruker TruLive3D light sheet microscope has t...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  Once the microscope, cameras and temperature controller and gas mixer are on it is time to open the LuxBundle software. Find the Luxendo logo on the taskbar and click on it. ...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  Calibration of the laser beams is critical for optimal imaging and should be done prior to every experiment. If you are using the temperature controller you should wait that t...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  This page will guide through the steps to set-up a basic experiment. 1. Setting up an experiment You have many possibilities to set-up an experiment with the Bruker TruLive3D...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  Data acquired on the Bruker TrLive3D will typically be very large, on the order of TeraBytes of data. The Luxendo computer has enough internal capacity to store up to ~20TB of ...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  The Bruker TruLive3D has a photo-manipulation (PM) feature that you can use to ablate specific areas of your sample. Calibration of the PM module Before performing any kind o...

Introduction In this guide you will learn how to identify what kind of detector is appropriate for your experiment, how to understand the main specifications of the two types of cameras you will encounter the most in biological imaging and relate them to your...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  Training Program  Bruker TruLive3D Light Sheet Microscope    Session 1 – Instrument and Operation Overview  Microscope Startup Procedure  How to turn the micro...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu   

After your session, you should backup your data from the Opera Phenix. There are two main file formats for you to export your data: 1) as a TIFF series for analysis using MATLAB, ImageJ/Fiji, other programs, or 2) as an Archive file (compatible with Harmony on...

Basics of laser scanning confocal A laser scanning confocal microscope produces high resolution images of 3D samples of up to 15 microns. It operates using pinholes on the laser beam path, blocking the out of focus light from the specimen. Images are acquired...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  This microscope offers several capabilities, it has two light sources and one camera. In addition to the laser system, you can use the X-cite LED in widefield mode. The camera ...

 For questions, comments or concerns please email us at biof-imaging@colorado.edu  When you are done imaging, remove your sample from the microscope and place it on a kimwipe or other paper towel if it has oil or water. If you used an oil objective, please r...

Getting the system ready If the microscope is off, turn on the microscope following the protocol taped to the wall: A.    Turn on the Laser Power Strip #1. a.    Turn on the appropriate laser boxes by turning the key to the ON position. Then press...

Microscope Controls To control the microscope stage, you will use the joystick. The central knob controls the X and Y directions, you can change the speed by twisting the top of it.  The two knobs on the sides of the joystick can be used to lower or raise th...

When you are done imaging, remove your sample from the microscope and place it on a kimwipe or other paper towel as it has microscope oil on the bottom. To clean the objective please refer to the instructions of Dr. Dragavon. Shut down the system according...