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OPM Data processing in Fiji

Microscope manuals Single Objective Light Sheet Microscope...

OPM Data The data generated by the OPM is in TIFF format. Whether you acquire a single image o...

Updated 13 hours ago by Evolene Premillieu

Using the OPM

Microscope manuals Single Objective Light Sheet Microscope...

The Single Objective Light Sheet Microscope is a custom-designed microscope and is a VERY sensiti...

Updated 13 hours ago by Evolene Premillieu

Bruker TruLive3D Data visualization in Fiji

Microscope manuals Bruker TruLive3D

The data generated by the Bruker TruLive3D contains a Fiji header named bdv.h5.  Fiji is an open...

Updated 13 hours ago by Evolene Premillieu

6. Bruker TruLive3D Experiment

Microscope manuals Bruker TruLive3D

This page will guide through the steps to set-up a basic experiment. 1. Setting up an experime...

Updated 13 hours ago by Evolene Premillieu

Bruker TruLive3D Sample preparation guidelines

Microscope manuals Bruker TruLive3D

This page contains some tips for sample preparation for the Bruker Trulive 3D. It is not exhausti...

Updated 13 hours ago by Evolene Premillieu

5. Bruker TruLive3D Finding the sample

Microscope manuals Bruker TruLive3D

Make sure you performed the calibration of the system before lowering your sample in the chambe...

Updated 3 weeks ago by Evolene Premillieu

Sample preparation for the OPM

Microscope manuals Single Objective Light Sheet Microscope...

Good news: Traditional sample preparation! The OPM sample holders are similar to that of other c...

Updated 1 month ago by Evolene Premillieu

Specifications and primary uses of the OPM

Microscope manuals Single Objective Light Sheet Microscope...

Single Objective Light Sheet Microscope   The Single Objective Light Sheet Microscope is also r...

Updated 1 month ago by Evolene Premillieu

Examples

Data Visualization Tools Fiji plugin for annotating movies with ...

Updated 1 month ago by Evolene Premillieu

Plugin installation

Data Visualization Tools Fiji plugin for annotating movies with ...

Updated 1 month ago by Evolene Premillieu

References

Data Visualization Tools Fiji plugin for annotating movies with ...

Updated 1 month ago by Evolene Premillieu

Bruker TruLive3D specifications and primary uses

Microscope manuals Bruker TruLive3D

Bruker TruLive3D main specifications The TruLive3D has 4 imaging channels at 405nm, 488nm, 561...

Updated 2 months ago by Evolene Premillieu

9. Bruker TruLive3D shut-down procedure

Microscope manuals Bruker TruLive3D

Once you are done with your experiment, please proceed with the following shut-down procedure: ...

Updated 2 months ago by Evolene Premillieu

Bruker TruLive3D Data management pipeline

Microscope manuals Bruker TruLive3D

Updated 2 months ago by Evolene Premillieu

8. Bruker TruLive3D Using the photo-manipulation (PM) feature

Microscope manuals Bruker TruLive3D

The Bruker TruLive3D has a photo-manipulation (PM) feature that you can use to ablate specific ...

Updated 2 months ago by Evolene Premillieu

7. Bruker TruLive3D Data management

Microscope manuals Bruker TruLive3D

Data acquired on the Bruker TrLive3D will typically be very large, on the order of TeraBytes of...

Updated 2 months ago by Evolene Premillieu

4. Bruker Trulive3D calibration

Microscope manuals Bruker TruLive3D

Calibration of the laser beams is critical for optimal imaging and should be done prior to ever...

Updated 2 months ago by Evolene Premillieu

3. Bruker TruLive3D Lux-Bundle overview

Microscope manuals Bruker TruLive3D

Once the microscope, cameras and temperature controller and gas mixer are on it is time to op...

Updated 2 months ago by Evolene Premillieu

Bruker TruLive3D emission filters and fluorophores spectrum

Microscope manuals Bruker TruLive3D

This page will help you select which filter combination you need for most commonly used fluorop...

Updated 2 months ago by Evolene Premillieu

2. Bruker TruLive3D microscope overview

Microscope manuals Bruker TruLive3D

a.   The Bruker TruLive3D is a dual sided illumination light sheet microscope. The dual light she...

Updated 2 months ago by Evolene Premillieu