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User Agreement

Facility Guidelines

BioFrontiers Advanced Light Microscopy Core (ALMC) User Agreement Form  This agreement outlines ...

Updated 4 hours ago by Erin Richards

Acquiring an image with channel triggering (rapid, sequential imaging)

Microscope manuals Nikon Experiment set-up and saving

Select the STORM lasers and select one of your desired channels in the OC panel. 2. Selec...

Updated 2 weeks ago by Evolene Premillieu

Acquiring a large image

Microscope manuals Nikon Experiment set-up and saving

Check the box in the Large Image tab. Adjust the number of fields to be stitched together. En...

Updated 2 weeks ago by Evolene Premillieu

Acquiring more than one channel

Microscope manuals Nikon Experiment set-up and saving

Check the box in the λ (wavelengths) tab. Add a new channel by clicking the checkbox under Opt...

Updated 2 weeks ago by Evolene Premillieu

Acquiring a z-stack

Microscope manuals Nikon Experiment set-up and saving

Check the box in the Z tab. Select a mode to define the z-stack: Define the top and bottom, sy...

Updated 2 weeks ago by Evolene Premillieu

Acquiring multiple points in (x,y) in the same file

Microscope manuals Nikon Experiment set-up and saving

Check the box in the XY tab. Add points as you move around your sample by clicking the checkbo...

Updated 2 weeks ago by Evolene Premillieu

Image acquisition and saving

Microscope manuals Nikon Experiment set-up and saving

The Nikon Elements software offers many options for acquiring and saving your data in the ND Acqu...

Updated 2 weeks ago by Evolene Premillieu

Acquiring a time-lapse image series

Microscope manuals Nikon Experiment set-up and saving

Check the box in the Time tab. Add a new time-lapse setting by clicking the checkbox under Pha...

Updated 2 weeks ago by Evolene Premillieu

Scheduling a meeting

Facility Guidelines

To schedule a meeting with any of our staff, please start by sending an email to biof-imaging@c...

Updated 2 weeks ago by Erin Richards

Nikon N-STORM - Specifications

Microscope manuals Nikon N-STORM

General information Microscope type: TIRF and super resolutionPurpose: STORM- or PALM-based supe...

Updated 3 weeks ago by Erin Richards

Recognizing the core

Facility Guidelines

It is essential that the staff and use of the BioFrontiers Advanced Light Microscopy Core is reco...

Updated 3 weeks ago by Erin Richards

Harmony Workstation Specifications

Microscope manuals Analysis Workstations

Workstation Harmony   Device Name Imaging-SC2017   Processor Intel Xeon CPU ...

Updated 1 month ago by Erin Richards

Aivia Analysis Workstation Specifications

Microscope manuals Analysis Workstations

Workstation Aivia and Cellpose   Device Name BIOF-BECK-EP   Processor Intel ...

Updated 1 month ago by Erin Richards

VS200 sample preparation tips

Microscope manuals Evident VS200 Slide Scanner

Advice for sample preparation   To facilitate imaging with the slide scanner VS200, here are a ...

Updated 1 month ago by Evolene Premillieu

Gaining facility access

Facility Guidelines

The workflow below describes how to gain access to the instrumentation and resources found with...

Updated 1 month ago by Evolene Premillieu

Nikon AXR - Overview

Microscope manuals Nikon AXR and NSPARC Laser Scanning Con...

Updated 1 month ago by Evolene Premillieu

Nikon AXR FRAP procedure

Microscope manuals Nikon AXR and NSPARC Laser Scanning Con...

This page is a brief introduction to doing FRAP experiments with the Nikon AXR or Nikon AXR NSPAR...

Updated 1 month ago by Evolene Premillieu

Evident VS200 Data Management Protocol

Microscope manuals Evident VS200 Slide Scanner

DATADRIVE1 (D:): The drive for active data acquisitions DATASTORAGE (F:): Temporary storage loca...

Updated 2 months ago by Evolene Premillieu

Data management and storage policy

Facility Guidelines

The ALMC does not store data or guarantee that data will be stored on your behalf. It is our poli...

Updated 2 months ago by Erin Richards

Imaris Workstation Specifications

Microscope manuals Analysis Workstations

Workstation Imaris   Device Name BIOF-IMAG-AYLS   Processor AMD Ryzen Thread...

Updated 2 months ago by Erin Richards