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33 total results found

Nikon NSTORM Specifications

Microscope manuals Nikon NSTORM

PerkinElmer Opera Phenix Specifications

Microscope manuals PerkinElmer Opera Phenix

General information Microscope type: Spinning disk confocal and widefieldPurpose: High-throughput and high-content imaging of multi-well plates will full automationFPbase link: https://www.fpbase.org/microscope/rBKJsuQSCGgSnZuxcGRYSh/ Equipment information ...

Molecular Devices ImageXpress Specifications

Microscope manuals Molecular Devices ImageXpress

Molecular Devices ImageXpress MicroXLS                     Widefield                     High Content Screening of multiwell plates                     Yes                     Yes          ...

Imaris Workstation Specifications

Microscope manuals Workstations

Workstation Imaris   Device Name Imaging-SC2017   Processor Intel Xeon CPU E5-2667 v4 @ 3.20GHz   RAM 256 GB   Operating System 64-Bit Windows 10   Graphics Card NVIDIA GeForce GTX 1080-Ti   Software Imaris v10...

Elements Workstations Specifications

Microscope manuals Workstations

Elements   AI-Analysis   Intel Core i7-47705 CPU @3.50 GHz   32 GB   Windows 7   NVIDIA GeForce GTX 690   NIS Elements v4.51.01 ImageJ/Fiji   Matlab r2020b

Basic guidelines

Image Analysis

The following are some suggested guidelines for handling your data. Please feel free to contact us if you have any questions about how to implement these. Backing up your data Back up your most important data (usually those related to a publication) to mul...

Exporting a video file

Microscope manuals PerkinElmer Opera Phenix

This document explains how to export a video file from a time-lapse dataset. Click on a well in imaging dataset Click on the Timepoints panel, then press CTRL+A to select all the timepoints Right-click on one of the selected timepoints and select "U...

Data management and storage policy

Facility Guidelines

The ALMC does not store data or guarantee that data will be stored on your behalf. It is our policy that you are personally responsible for your data stored on our computers. It is therefore your responsible to transfer data and maintain your own backups.  Pl...

Image Analysis Workflow

Image Analysis

This page details the workflow for service request. Note that all requests for service MUST be submitted through our ticketing system. Send an email to biof-imaging@colorado.edu with a brief description of your request. If you request is for a new project,...

Scheduling a meeting

Facility Guidelines

To schedule a meeting with any of our staff, please start by sending an email to biof-imaging@colorado.edu. You will receive an automated reply that contains a link to our Microsoft Bookings page. The email should be sent as soon as the system receives your...

Getting started with Imaris

Image Analysis Imaris

To load an image in Imaris Make sure you are in the Arena view (see above). Click on Observe Folder, then select the folder with your images in the dialog box that pops up. If you have not opened the file before, the file will likely have an ND2 extensi...

Intensity-based thresholding

Image Analysis Imaris

This page explains how to use intensity-based thresholding (the traditional method) to segment cells. For this example, we will segment some nuclei which have been labeled with DAPI. In the Surpass view, click on the Surfaces icon in the left panel to creat...

Machine learning segmentation

Image Analysis Imaris

This section explains how to use the ML segmentation tool in Imaris.  In the Surpass view, click on the Surfaces icon in the left panel to create a new Surface. A wizard dialog box will appear on the bottom left of the screen. On this page:  Unselect C...