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Nikon NSTORM Specifications
PerkinElmer Opera Phenix Specifications
General information Microscope type: Spinning disk confocal and widefieldPurpose: High-throughput and high-content imaging of multi-well plates will full automationFPbase link: https://www.fpbase.org/microscope/rBKJsuQSCGgSnZuxcGRYSh/ Equipment information ...
Molecular Devices ImageXpress Specifications
Molecular Devices ImageXpress MicroXLS Widefield High Content Screening of multiwell plates Yes Yes ...
Imaris Workstation Specifications
Workstation Imaris Device Name Imaging-SC2017 Processor Intel Xeon CPU E5-2667 v4 @ 3.20GHz RAM 256 GB Operating System 64-Bit Windows 10 Graphics Card NVIDIA GeForce GTX 1080-Ti Software Imaris v10...
Elements Workstations Specifications
Elements AI-Analysis Intel Core i7-47705 CPU @3.50 GHz 32 GB Windows 7 NVIDIA GeForce GTX 690 NIS Elements v4.51.01 ImageJ/Fiji Matlab r2020b
Basic guidelines
The following are some suggested guidelines for handling your data. Please feel free to contact us if you have any questions about how to implement these. Backing up your data Back up your most important data (usually those related to a publication) to mul...
Exporting a video file
This document explains how to export a video file from a time-lapse dataset. Click on a well in imaging dataset Click on the Timepoints panel, then press CTRL+A to select all the timepoints Right-click on one of the selected timepoints and select "U...
Data management and storage policy
The ALMC does not store data or guarantee that data will be stored on your behalf. It is our policy that you are personally responsible for your data stored on our computers. It is therefore your responsible to transfer data and maintain your own backups. Pl...
Image Analysis Workflow
This page details the workflow for service request. Note that all requests for service MUST be submitted through our ticketing system. Send an email to biof-imaging@colorado.edu with a brief description of your request. If you request is for a new project,...
Scheduling a meeting
To schedule a meeting with any of our staff, please start by sending an email to biof-imaging@colorado.edu. You will receive an automated reply that contains a link to our Microsoft Bookings page. The email should be sent as soon as the system receives your...
Getting started with Imaris
To load an image in Imaris Make sure you are in the Arena view (see above). Click on Observe Folder, then select the folder with your images in the dialog box that pops up. If you have not opened the file before, the file will likely have an ND2 extensi...
Intensity-based thresholding
This page explains how to use intensity-based thresholding (the traditional method) to segment cells. For this example, we will segment some nuclei which have been labeled with DAPI. In the Surpass view, click on the Surfaces icon in the left panel to creat...
Machine learning segmentation
This section explains how to use the ML segmentation tool in Imaris. In the Surpass view, click on the Surfaces icon in the left panel to create a new Surface. A wizard dialog box will appear on the bottom left of the screen. On this page: Unselect C...