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Exporting and deleting files
After your session, you should backup your data from the Opera Phenix. There are two main file formats for you to export your data: 1) as a TIFF series for analysis using MATLAB, ImageJ/Fiji, other programs, or 2) as an Archive file (compatible with Harmony on...
Nikon A1R - Overview
Basics of laser scanning confocal A laser scanning confocal microscope produces high resolution images of 3D samples of up to 15 microns. It operates using pinholes on the laser beam path, blocking the out of focus light from the specimen. Images are acquired...
Nikon SDC - Mode of operation
This microscope offers several capabilities, it has two light sources and one camera. In addition to the laser system, you can use the X-cite LED in widefield mode. The camera installed is an EMCCD iXon 888 with 13 µm pixels in a 1024 x 1024 format. Micro...
Nikon SDC - Shut-down and clean up
When you are done imaging, remove your sample from the microscope and place it on a kimwipe or other paper towel if it has oil or water. If you used an oil objective, please refer to the instructions of Dr. Dragavon to clean it: Shut down the system accor...
Nikon NSTORM - Basic Set-up
Getting the system ready If the microscope is off, turn on the microscope following the protocol taped to the wall: A. Turn on the Laser Power Strip #1. a. Turn on the appropriate laser boxes by turning the key to the ON position. Then press...
Nikon NSTORM - Mode of operation
Microscope Controls To control the microscope stage, you will use the joystick. The central knob controls the X and Y directions, you can change the speed by twisting the top of it. The two knobs on the sides of the joystick can be used to lower or raise th...
Nikon NSTORM - Shut-down and clean up
When you are done imaging, remove your sample from the microscope and place it on a kimwipe or other paper towel as it has microscope oil on the bottom. To clean the objective please refer to the instructions of Dr. Dragavon. Shut down the system according...
Using the Perfect Focus System (PFS)
The perfect focus system locates the coverslip and continually adjusts the objective height to follow any drift in the system. Engaging the PFS Only if using the 100x oil objective, first raise the objective by turning the z-axis control until the oil cont...
Using the Perfect Focus System (PFS)
The perfect focus system locates the coverslip and continually adjusts the objective height to follow any drift in the system. Engaging the PFS Only if using the 100x oil objective, first raise the objective by turning the z-axis control until the oil co...
Shut Down and clean up procedures
When you are done imaging, remove your sample from the microscope and place it on a kimwipe or other paper towel if it has oil or water. If you used an oil objective, please refer to the instructions of Dr. Dragavon to clean it. Shut down the system accordi...
9. Bruker TruLive3D shut-down procedure
Once you are done with your experiment, please proceed with the following shut-down procedure: Turn off the microscope Turn off the cameras Turn off the chiller Turn off the gas mixer controller Close the CO2 valves Remove y...
Data management and storage policy
The ALMC does not store data or guarantee that data will be stored on your behalf. It is our policy that you are personally responsible for your data stored on our computers. It is therefore your responsible to transfer data and maintain your own backups. Pl...
PerkinElmer - Opera Phenix manual
This microscope has excellent online resources that you can consult after completing your in-person training. The University of Colorado PerkinElmer Opera Phenix training site can be found here. To login, please email Joe Dragavon at biof-imaging@colorado....
5. Bruker TruLive3D Finding the sample
Make sure you performed the calibration of the system before lowering your sample in the chamber. Finding your sample You should have already installed your sample, so the first step is to lower the z stage to bring the sample between the two illumin...
Online learning resources by Nikon
Nikon is now offering learning resources available online. To request a login password, please email Joe Dragavon at biof-imaging@colorado.edu Nikon e-learning center "The Nikon Instruments Learning Center provides interactive tutorials on a variety of topic...
Online learning resources by Nikon
Nikon is now offering learning resources available online. To request a login password, please email Joe Dragavon at biof-imaging@colorado.edu Nikon e-learning center "The Nikon Instruments Learning Center provides interactive tutorials on a variety of top...
Online learning resources by Nikon
Nikon is now offering learning resources available online. To request a login password, please email Joe Dragavon at biof-imaging@colorado.edu Nikon e-learning center "The Nikon Instruments Learning Center provides interactive tutorials on a variety of topic...
Online learning resources by Nikon
Nikon is now offering learning resources available online. To request a login password, please email Joe Dragavon at biof-imaging@colorado.edu Nikon e-learning center "The Nikon Instruments Learning Center provides interactive tutorials on a variety of topic...
Image Analysis Workflow
This page details the workflow for service request. Note that all requests for service MUST be submitted through our ticketing system. Send an email to biof-imaging@colorado.edu with a brief description of your request. If you request is for a new project,...
Scheduling a meeting
To schedule a meeting with any of our staff, please start by sending an email to biof-imaging@colorado.edu. You will receive an automated reply that contains a link to our Microsoft Bookings page. The email should be sent as soon as the system receives your...