Specifications
Basic Information
| Microscope | Nikon Widefield |
| Type | Widefield |
| Purpose | Rapid and long-term 2D imaging |
| Fixed Cells | Yes |
| Live Cells | Yes |
| FPbase | https://www.fpbase.org/microscope/hkGFJUe7zVWYEuD4ev85ie/ |
| Tag | HHMI 301492 |
| HHMI 311898 | |
| Room | C305D |
| Software | NIS Elements v5.20.02 |
| HASP | 26FCA24 |
| Base | Nikon TiE |
| XY Stage | Motorized |
| Z | Ti Z drive |
| Autofocus | PFS 3 |
| Environment Control | Oko Labs enclosure: CO2, Humidity, Temperature |
| Light Source | Excelitas Xylis LED |
Filters
| Excitation | Emission | ||||||||
| Center | Width | Min | Max | Center | Width | Min | Max | Fluorophores | Vendor |
| 350 | 50 | 325 | 375 | 460 | 50 | 435 | 485 | DAPI, BFP | Chroma |
| 470 | 40 | 450 | 490 | 525 | 50 | 500 | 550 | GFP, FITC, Alexa Fluor 488 | Chroma |
| 560 | 40 | 540 | 580 | 630 | 75 | 592.5 | 667.5 | TRITC, Texas Red, dsRed | Dichroic T585lpxr |
| 620 | 60 | 590 | 650 | 700 | 75 | 662.5 | 737.5 | Cy5, Alexa Fluor 647, Draq5 | Chroma |
Objectives
| Model | Magnification | NA | Immersion |
| 4 | 0.2 | Air | |
| 10 | 0.45 | Air | |
| 20 | 0.75 | Air | |
| 40 | 0.95 | Air | |
| 100 | 1.4 | Oil |
Detectors
| sCMOS |
| Hamamatsu Flash 4.0 v3 |
| S/N: 170216-SYS-006488 |