Nikon Widefield Specifications

BasicGeneral Information

Microscope

Purpose:
FPbase link:

Equipment

information

Location:

Sample

types

Fixed

cells

Microscope	Nikontype: Widefield
Type	Widefield
Purpose	Rapid and long-term 2D imaging
Fixed Cells	Yes
Live Cells	Yes
FPbase	https://www.fpbase.org/microscope/hkGFJUe7zVWYEuD4ev85ie/
Tag	JSCBB C305D Equipment tags: HHMI 301492
	HHMI 311898
Room	C305D

Live cells

Equipment information

Software	NIS Elements v5.20.02
HASP	26FCA24
Base	Nikon TiE
XY Stage	Motorized
Z	Ti Z drive
Autofocus	PFS 3
Environment Control	Oko Labs enclosure: CO2, Humidity, Temperature
Light Source	Excelitas Xylis LED

Filters Cubes

				Excitation								Emission
	Center		Width		Min		Max		Center		Width		Min		Max		Fluorophores		Vendor
350	50	325	375	460	50	435	485	DAPI, BFP	Chroma
470	40	450	490	525	50	500	550	GFP, FITC, Alexa Fluor 488	Chroma
560	40	540	580	630	75	592.5	667.5	TRITC, Texas Red, dsRed	Dichroic T585lpxr
620	60	590	650	700	75	662.5	737.5	Cy5, Alexa Fluor 647, Draq5	Chroma

Objectives

Model	Magnification	NA	Immersion
	4	0.2	Air
	10	0.45	Air
	20	0.75	Air
	40	0.95	Air
	100	1.4	Oil

Detectors

sCMOS

Hamamatsu Flash 4.0 v3

S/N: 170216-SYS-006488