A. Bruker TruLive3D emission filters and fluorophores spectrum

This page will help you ~~figure out~~select which filter combination you need for most commonly used fluorophores on the Bruker TruLive3D.

The Bruker TruLive3D light sheet microscope has two imaging cameras with two motorized filter wheels and two sets of filters. It is designed to split different emission wavelengths between the two cameras for simultaneous imaging with two different emission channels.

The "Long Camera" will record the fluorescence emitting at the longest wavelength and the "Short Camera" will record the fluorescence emitting at the shortest wavelength.

When you set up your experiment you want to make sure that you select the best filter option for each camera so that the fluorescence from one fluorophore does not mix up with the other.

The Bruker TruLive3D has the following emission filters options:

Filter wheel Short Camera: BP418-462 LP498 BP497-554 BP580-627 BP610-651

Filter wheel Long Camera: LP498 BP497-554 BP580-627 BP610-651 BP655-704

Below is a list of the most commonly used fluorophores and the filters you should use:

Fluorophores	Filter	Laser line
GFP	BP497-554	488
DAPI	BP418-462	405
TRITC	BP580-627	561
mCherry	BP580-627	561
AlexaFluor488	BP497-554	488
AlexaFluor555	BP580-627	488 or 561
AlexaFluor594	BP580-627 or BP610-651	405
AlexaFluor647	BP655-704	640

To select the appropriate filters you need to know the emission peak wavelength of the fluorophores you are using.

~~Below is~~For a ~~list~~better ofunderstanding on how to select the ~~most~~correct ~~commonly~~filter, ~~used~~you ~~fluorophores~~can ~~and~~look aat ~~screenshot of their~~the excitation/emission spectrums from fpbase.org or aatbio.com .for example. When you click on the link you can use the interactive spectrum ~~viewer.~~viewer and see what the filters cover.