| Microscope: |
PerkinElmer Opera Phenix |
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| Type |
Spinning Disc Confocal and Widefield |
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| Purpose |
High-throughput High-content imaging of multiwell plates will full automation |
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| Fixed Cells |
Yes |
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| Live Cells |
Yes |
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| FPbase |
https://www.fpbase.org/microscope/rBKJsuQSCGgSnZuxcGRYSh/ |
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| S/N |
1400L18161 |
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| Tag |
CU |
UCB 214288 RC16 |
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| Room |
JSCBB |
C350C |
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| Software |
Harmony |
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v4.9 |
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| Autofocus |
Infrared |
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| Environment Control |
CO2, Humidity, Temperature |
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| Scan Head |
Modified spinning disc |
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| Light Source |
Lasers |
375 |
425 |
488 |
561 |
640 |
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| Filters |
Emission |
Center |
Width |
Min |
Max |
Fluorophores |
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Vendor |
Number |
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457.5 |
45 |
435 |
480 |
DAPI, BFP, CFP |
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Semrock |
FF01-457/50 |
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492.5 |
115 |
435 |
550 |
DAPI, BFP, CFP |
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475 |
80 |
435 |
515 |
DAPI, BFP, CFP |
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525 |
50 |
500 |
550 |
GFP, Alexa Fluor 488, FITC |
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Chroma |
ET525/50m |
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600 |
60 |
570 |
630 |
TRITC, Texas Red, dsRed, Alexa Fluor 568 |
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Omega |
600BP60 RapidBand |
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705 |
110 |
650 |
760 |
Cy5, Alexa Fluor 647, Draq5 |
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| Objectives |
Magnification |
NA |
Immersion |
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5 |
0.16 |
Air |
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10 |
0.3 |
Air |
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20 |
0.8 |
Air |
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20 |
1 |
Water |
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40 |
1.1 |
Water |
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| Detectors |
sCMOS |
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x4 Andor Xyla |
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