Environment Control |
CO2, Humidity, Temperature
Laser lines
- 375 nm
- 425 nm
- 488 nm
- 561 nm
- 640 nm
Filters
| Center (nm) |
Width (nm) |
Min (nm) |
Max (nm) |
Example fluorophores |
Vendor |
Model |
|
|
|
number |
|
Scan Head |
Modified spinning disc |
|
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|
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|
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|
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|
Light Source |
Lasers |
375 |
425 |
488 |
561 |
640 |
|
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|
Filters |
Emission |
Center |
Width |
Min |
Max |
Fluorophores |
|
|
Vendor |
Number |
|
|
|
457.5 |
45 |
435 |
480 |
DAPI, BFP, CFP |
|
|
Semrock |
FF01-457/50 |
|
|
|
492.5 |
115 |
435 |
550 |
DAPI, BFP, CFP |
|
|
|
|
|
|
|
475 |
80 |
435 |
515 |
DAPI, BFP, CFP |
|
|
|
|
|
|
|
525 |
50 |
500 |
550 |
GFP, Alexa Fluor 488, FITC |
|
|
Chroma |
ET525/50m |
|
|
|
600 |
60 |
570 |
630 |
TRITC, Texas Red, dsRed, Alexa Fluor 568 |
|
|
Omega |
600BP60 RapidBand |
|
|
|
705 |
110 |
650 |
760 |
Cy5, Alexa Fluor 647, Draq5 |
|
|
Objectives
Magnification |
NA |
Immersion medium |
|
Objectives |
Magnification |
NA |
Immersion |
|
|
|
|
|
|
|
|
|
5 |
0.16 |
Air |
|
|
|
|
|
|
|
|
|
10 |
0.3 |
Air |
|
|
|
|
|
|
|
|
|
20 |
0.8 |
Air |
|
|
|
|
|
|
|
|
|
20 |
1 |
Water |
|
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|
|
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|
|
|
|
40 |
1.1 |
Water |
|
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Detectors |
sCMOS |
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x4 Andor Xyla |
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Detectors
- Andor Xyla x4 sCMOS camera
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