PerkinElmer Opera Phenix Specifications
General information
Microscope type: Spinning disk confocal and widefield
Purpose: High-throughput and high-content imaging of multi-well plates will full automation
FPbase link: https://www.fpbase.org/microscope/rBKJsuQSCGgSnZuxcGRYSh/
Equipment information
Location: JSCBB C350C
Serial number: 1400L18161
CU Tag: UCB 214288 RC16
Sample types
| Fixed Cells | Yes |
| Live Cells | Yes |
Software
- Harmony v4.9
Environmental control
- CO2, Humidity, Temperature
Laser lines
- 375 nm
- 425 nm
- 488 nm
- 561 nm
- 640 nm
Filters
| Center (nm) | Width (nm) | Min (nm) | Max (nm) | Example fluorophores | Vendor | Model number |
| 457.5 | 45 | 435 | 480 | DAPI, BFP, CFP | Semrock | FF01-457/50 |
| 492.5 | 115 | 435 | 550 | DAPI, BFP, CFP | ||
| 475 | 80 | 435 | 515 | DAPI, BFP, CFP | ||
| 525 | 50 | 500 | 550 | GFP, Alexa Fluor 488, FITC | Chroma | ET525/50m |
| 600 | 60 | 570 | 630 | TRITC, Texas Red, dsRed, Alexa Fluor 568 | Omega | 600BP60 RapidBand |
| 705 | 110 | 650 | 760 | Cy5, Alexa Fluor 647, Draq5 |
Objectives
| Magnification | NA | Immersion medium |
| 5 | 0.16 | Air |
| 10 | 0.3 | Air |
| 20 | 0.8 | Air |
| 20 | 1 | Water |
| 40 | 1.1 | Water |
Detectors
- Andor Xyla x4 sCMOS camera