Nikon SDC Specifications
Type: Spinning disc confocal
Purpose: Rapid 2D and 3D imaging of samples
Fixed cells: Yes
Live cells: Yes
FPBase link: https://www.fpbase.org/microscope/X6ne45wr4DYFPsgc5nPrKo
Tag: None
Room: C350A
Software:
- NIS Elements - v5.42.03
HASP: 293CDCAA
Base: Nikon TiE
XY Stage: Motorized
Z:
- Ti Z drive
- MCL Piezo Z - 100 µm
Autofocus: PFS 3
Environment control:
- Oko Labs enclosure: CO2, Humidity, Temperature
Scan head: Yokogawa CSU-X1
Light source:
- LUNV22 70186
- Lasers: 405, 445, 488, 515, 561, 594, 640
Emission Filters
| Vendor | Model | Center | Width | Min | Max | Common Fluorophores |
| Chroma | ETDAPI-Cy5 m Lot 314725 | 437 | 18 | 428 | 446 | DAPI. BFP |
| Chroma | ET482/27m | 482 | 34 | 465 | 499 | CFP |
| Chroma | ET525/50m | 525 | 50 | 500 | 550 | GFP, FITC, Alexa Fluor 488 |
| Chroma | ET540/30m | 540 | 30 | 525 | 555 | YFP |
| Chroma | ET620/60m | 620 | 60 | 590 | 650 | TRITC, Texas Red, dsRed, Alexa Fluor 568 |
| Chroma | ET630/30m | 630 | 30 | 615 | 645 | mCherry |
| Chroma | ETDAPI-Cy5 m Lot 314725 | 705 | 68 | 671 | 739 | Cy5, Alexa Fluor 647, Draq5 |
Dichroics
- Chroma ZT405/488/561/640rpcv2
- Chroma ZT44/514/594rpc
Objectives
| Magnification | NA | Medium |
| 4 | 0.2 | Air |
| 10 | 0.45 | Air |
| 20 | 0.75 | Air |
| 40 | 0.95 | Air |
| 100 | 1.4 | Oil |
Detectors
- EMCCD
- Andor IXon 888 Ultra
- S/N: X-9963