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Basic use

This protocol covers the basics of turning on the microscope, mounting your sample, and acquiring a simple image.

  1. If the microscope is off, turn on the microscope following (the protocol is also taped toon the wall in the facility):

     


 


  1. Turn on the lasers and microscope using the remote that is attached on the wall. Turn on the heat for the environmental chamber if needed.  


 

  1. Turn on the laser lines that you need by pressing the buttons and turn off the laser lines that you don’t need. 


 

 

 

 

 

 

 


  1. Insert the sample plate holder 


 

Depending on your experiment you may choose a different plate. You can turn on the light in the environmental chamber using the pedal switch on the floor under the microscope. The intensity can be adjusted with the white knob on top of the environmental chamber. If there are any oil stains on the equipment please clean up with ethanol before starting your experiment. The following figure shows the steps to look at a sample slide. Remember to always place the coverslip towards the objective. 

 

 
 


  1. Nikon NDC microscope features 


 

This microscope offers several capabilities, it has two light sources and one camera. In addition to the laser system, you can use the X-cite LED in widefield mode. The camera installed is an EMCCD iXon 888 with 512x512 pixels. 

 
 

  1. Click on the Nikon Elements icon on the desktop. Then click ok for iXon 888. 
     

  1. Click OK when the dialog box appears saying “Optical Configurations Import successfully finished.” 


 

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