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Bruker TruLive3D training protocol

 For questions, comments or concerns please email us at biof-imaging@colorado.edu 


Training Program 

Bruker TruLive3D Light Sheet Microscope 

 

Session 1 – Instrument and Operation Overview 

Microscope Startup Procedure 

  1. How to turn the microscope and computer on 

Microscope overview 

  1. What are the parts of the microscope and what is each one for 

Optical Path Overview 

  1. Illumination + Collection objectives 

  1. Define XYZ directions 

  1. Using the space mouse to control the translation stage 

Bruker Lux-Bundle Overview  

  1. Image Viewing Area 

  1. Calibration 

  1. Dashboard 

  1. Image Viewer 

  1. Image Processing 

Microscope calibration 

  1. Align the two beams in water 

  1. Perform the line calibration 

Session 2 – Setting up an experiment 

Review of microscope calibration 

  1. Align the two beams in water 

  1. Perform the line calibration 

Sample finding 

  1. Load your sample in the chamber 

  1. Find your sample with the BlackFly camera on the SpinView software 

  1. Choose an area of your sample with the detection cameras  

Setting up an experiment in LuxBundle 

  1. Set up different channels 

  1. Set up z-stacks and scan areas 

  1. Set up a trigger 

Data saving options 

  1. Write your data locally and then delete it 

  1. Write your data directly on your server 

  1. Fill out the book-keeping form with your data identification


 Data analysis in LuxBundle 

     1. Image viewing in LuxBundle

     2. Image processing in LuxBundle: learn how to create separate imaris headers for each stack

 Shutdown Procedure and Calendar Access 

     1. System shutdown 

     2. Cleaning procedure 

     3. Calendar access 

 

Session 3 – User-led Session 


  1. Reserve time on the microscope 

     2. Notify the Instructor as to the date/time of the session 

     3. Reserve at least two hours 

     4. Turn on the microscope 

     5. Load your sample 

     6. Calibrate the instrument 

     7. Find your sample 

     8. Set up an experiment 

The instructor will check in on you throughout to offer assistance 

Optional Session – Photomanipulation module 

 

  1. Review of Session 1 

     2. Beam Calibration 

     3. Photo-Manipulation Module 

     4. Calibration of the PM module 

     5. Load in your sample 

     6. Find your sample on the SpinView software with the BlackFly camera 

     7. Explore the different photomanipulation options 

     8. Set up an experiment with a photomanipulation event 


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