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Overview of the OC (Optical Configuration) control panel  The OC Panel contains the different light source configurations. You will principally use the Nikon A1 configuration for the laser scanning with the Nikon PMT as a detector. The DIA (diascopic transmit...

General information Microscope type: Spinning disk confocal and widefieldPurpose: High-throughput and high-content imaging of multi-well plates will full automationFPbase link: https://www.fpbase.org/microscope/rBKJsuQSCGgSnZuxcGRYSh/ Equipment information ...

Molecular Devices ImageXpress MicroXLS                     Widefield                     High Content Screening of multiwell plates                     Yes                     Yes          ...

Workstation Imaris   Device Name Imaging-SC2017   Processor Intel Xeon CPU E5-2667 v4 @ 3.20GHz   RAM 256 GB   Operating System 64-Bit Windows 10   Graphics Card NVIDIA GeForce GTX 1080-Ti   Software Imaris v10...

Elements   AI-Analysis   Intel Core i7-47705 CPU @3.50 GHz   32 GB   Windows 7   NVIDIA GeForce GTX 690   NIS Elements v4.51.01 ImageJ/Fiji   Matlab r2020b

The following are some suggested guidelines for handling your data. Please feel free to contact us if you have any questions about how to implement these. Backing up your data Back up your most important data (usually those related to a publication) to mul...

  a.      Turn on the microscope In case of emergency, push the big red button: it turns off the entire system. When you turn it back on, turn it counter clockwise until it is pulled all the way. b.      Turn on the two cameras c.      Fill the sample ...

a.   The Bruker TruLive3D is a dual sided illumination light sheet microscope. The dual light sheet is projected on the sample by a pair of water immersion 10X 0.3NA microscope objectives. The detection objective is a water immersion 25X 1.1NA objective. An ad...

This page will help you select which filter combination you need for most commonly used fluorophores on the Bruker TruLive3D. The Bruker TruLive3D light sheet microscope has two imaging cameras with two motorized filter wheels and two sets of filters. It is...

Once the microscope, cameras and temperature controller and gas mixer are on it is time to open the LuxBundle software. Find the Luxendo logo on the taskbar and click on it. If you are using the temperature controller the water bath in the chamber wil...

Calibration of the laser beams is critical for optimal imaging and should be done prior to every experiment. If you are using the temperature controller you should wait that the temperature in the sample chamber is close to the set value to perform the cali...

This page will guide through the steps to set-up a basic experiment. 1. Setting up an experiment You have many possibilities to set-up an experiment with the Bruker TruLive3D. You can record a single z-stack at several time points, multiple z-stacks at sev...

Data acquired on the Bruker TrLive3D will typically be very large, on the order of TeraBytes of data. The Luxendo computer has enough internal capacity to store up to ~20TB of data. This seems like a lot of storage but we are asking the users to transfer the...

The Bruker TruLive3D has a photo-manipulation (PM) feature that you can use to ablate specific areas of your sample. Calibration of the PM module Before performing any kind of photo-manipulation, the PM module needs to be calibrated.  Click on the Calibra...

This document explains how to export a video file from a time-lapse dataset. Click on a well in imaging dataset Click on the Timepoints panel, then press CTRL+A to select all the timepoints Right-click on one of the selected timepoints and select "U...

Introduction In this guide you will learn how to identify what kind of detector is appropriate for your experiment, how to understand the main specifications of the two types of cameras you will encounter the most in biological imaging and relate them to your...

Training Program  Bruker TruLive3D Light Sheet Microscope    Session 1 – Instrument and Operation Overview  Microscope Startup Procedure  How to turn the microscope and computer on  Microscope overview  What are the parts o...