VS200 - Specifications
General information
Microscope type: Widefield slide scanner
Purpose: Rapid semi-automated scanning of fixed samples on slides
FPbase link: https://www.fpbase.org/microscope/ip8R2V8YiDaHCBa9hJmP87/
Equipment information
Location: JSCBB C1B76
CU Tag: UCB 218991
Sample types
| Fixed Cells | Yes |
| Live Cells | Not recommended, no environmental control |
Software
- Olympus CellSens
Environmental control
- None
Light source
- X-Cite NOVEM-L XT920
Filters
| Excitation | Emission | Fluors |
||||||
| Center | Width | Min | Max | Center | Width | Min | Max | |
| 387.5 | 11 | 382 | 393 | 440 | 40 | 420 | 460 | DAPI, CellTrace Blue |
| 485 | 20 | 475 | 495 | 521 | 21 | 510 | 531 | FITC, Alexa 488 |
| 560 | 25 | 548 | 573 | 607 | 34 | 589.5 | 623.5 | TRITC, Alexa 568, Nile Red |
| 650 | 13 | 644 | 657 | 694 | 34.5 | 677 | 711.5 | Cy5, Alexa 647, Opal 690 |
| 740 | 13 | 734 | 747 | 809 | 81 | 768.5 | 849.5 | Cy7, Opal 780 |
Objectives
| Magnification | NA | Immersion medium |
| 2X | 0.10 | Air |
| 10X | 0.40 | Air |
| 20X | 0.75 | Air |
| 40X | 0.95 | Air |
| 60X | 1.4 | Oil |
Detectors
- Hamamatsu Fusion Gen-III
- 16-bit detectors: pixel intensity values from 0 - 65,535
- Nikon DS-Fi3 (RGB color camera)
- 8-bit detector: pixel intensity values from 0 - 255
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