# PerkinElmer Opera Phenix Specifications ### General information **Microscope type:** Spinning disk confocal and widefield **Purpose:** High-throughput and high-content imaging of multi-well plates will full automation **FPbase link:** [https://www.fpbase.org/microscope/rBKJsuQSCGgSnZuxcGRYSh/](https://www.fpbase.org/microscope/rBKJsuQSCGgSnZuxcGRYSh/) ### Equipment information **Location:** JSCBB C350C **Serial number:** 1400L18161 **CU Tag:** UCB 214288 RC16 ### Sample types

Fixed Cells	Yes
Live Cells	Yes

### Software - Harmony v4.9 ### Environmental control - CO2, Humidity, Temperature ### Laser lines - 375 nm - 425 nm - 488 nm - 561 nm - 640 nm ### Filters

**Emission				Example fluorophores**	Vendor**	Model number**
Center (nm)	Width (nm)	Min (nm)	Max (nm)	Example fluorophores**	Vendor**	Model number**
457.5	45	435	480	DAPI, BFP, CFP	Semrock	FF01-457/50
492.5	115	435	550	DAPI, BFP, CFP
475	80	435	515	DAPI, BFP, CFP
525	50	500	550	GFP, Alexa Fluor 488, FITC	Chroma	ET525/50m
600	60	570	630	TRITC, Texas Red, dsRed, Alexa Fluor 568	Omega	600BP60 RapidBand
705	110	650	760	Cy5, Alexa Fluor 647, Draq5

### Objectives

Magnification	NA	Immersion medium
5	0.16	Air
10	0.3	Air
20	0.8	Air
20	1	Water
40	1.1	Water

### Detectors - Andor Xyla x4 sCMOS camera