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PerkinElmer Opera Phenix Specifications

General information

Microscope type: Spinning disk confocal and widefield
Purpose: High-throughput and high-content imaging of multi-well plates will full automation
FPbase link: https://www.fpbase.org/microscope/rBKJsuQSCGgSnZuxcGRYSh/

Equipment information

Location: JSCBB C350C

Serial number: 1400L18161
CU Tag: UCB 214288 RC16

Sample types

Fixed Cells Yes
Live Cells Yes

Software

Environmental control

Laser lines

Filters

Emission

Example fluorophores

Vendor

Model number
Center (nm) Width (nm) Min (nm) Max (nm)
457.5 45 435 480 DAPI, BFP, CFP Semrock FF01-457/50
492.5 115 435 550 DAPI, BFP, CFP    
475 80 435 515 DAPI, BFP, CFP    
525 50 500 550 GFP, Alexa Fluor 488, FITC Chroma ET525/50m
600 60 570 630 TRITC, Texas Red, dsRed, Alexa Fluor 568 Omega 600BP60 RapidBand
705 110 650 760 Cy5, Alexa Fluor 647, Draq5    

Objectives

Magnification NA Immersion medium
5 0.16 Air
10 0.3 Air
20 0.8 Air
20 1 Water
40 1.1 Water

Detectors


Revision #4
Created 28 July 2023 18:16:33 by Jian Tay
Updated 9 February 2024 20:19:21 by Jian Tay