Nikon NSPARC - Specifications

General information

Microscope type: Laser scanning confocal
Purpose: High quality 2D and 3D images of fixed and live samples using a particularly sensitive detector for dim or small objects
FPbase link: https://www.fpbase.org/microscope/3KjeQgHxQ75zPDd372PTuc/

Equipment information

Location: JSCBB C350D

CU Tag: UCB 218848

Sample types

Fixed Cells	Yes
Live Cells	Yes

Software

• NIS Elements 6.10.01

Base

• Nikon Ti2-E

Environmental control

• CO₂, Humidity, Temperature

Laser lines

• 405 nm
• 445 nm
• 488 nm
• 515 nm
• 561 nm
  
• 640 nm
  

Filters

Emission				Example fluorophores
Center (nm)	Width (nm)	Min (nm)	Max (nm)
446.5	57	418	475	DAPI, BFP, CFP
452.5	45	430	475
482.5	35	465	500	CFP
524	44	502	546	GFP, Alexa 488
540	30	525	555	YFP
593	45	570	616	TRITC, Alexa 568, mCherry
699.5	75	662	737	Cy5, Alexa 647, Draq5

Objectives

Magnification	NA	Immersion medium
10X	0.45	Air
20X	0.75	Air
20X	0.95	Water
40X	1.25	Silicone
60X	1.49	Oil
100X	1.45	Oil

Detectors

• NSPARC with GaAsp detectors for all laser lines
  • 12-bit detectors: pixel intensity values from 0 - 4095

More information about the NSPARC detector can be found here.