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Nikon N-STORM - Specifications

General information

Microscope type: TIRF and super resolution
Purpose: STORM- or PALM-based super resolution, TIRF, HiLo, or FRAP
FPbase link: https://www.fpbase.org/microscope/p8tJqsbQSCuVeuQvhbrAM3/

Equipment information

Location: JSCBB C350B

Sample types

Fixed Cells Yes
Live Cells Yes

Software

  • NIS Elements 6.2

Base

  • Nikon Ti2-E

Environmental control

  • CO2, Humidity, Temperature

Laser lines

TIRF/STORM

  • 405 nm
  • 488 nm
  • 561 nm
  • 647 nm

FRAP

  • 405 nm
  • 488 nm
  • 561 nm
  • 640 nm

Filters

Dichroic Mirror (for Emission)
 Example fluorophores
Center (nm) Width (nm) Min (nm) Max (nm)
450 60 420 480 DAPI, BFP, CFP

525

 60 495 555 GFP, FITC, Alex 488
600 50 575 625 TRITC, Texas Red, Alex 568
727.5 125 665 790 Cy5, Alexa 647, Draq5

Objectives

Magnification NA Immersion medium
10X 0.45 Air
20X 0.75 Air
60X TIRF 1.49 Oil
100X TIRF 1.49 Oil

Detectors

  • Two Andor iXon 897 (EMCCD) cameras equipped with TwinCam for simultaneous imaging of two colors
    • 16-bit detector: pixel intensities range from 0- 65,535
  • One Hamamatsu Orca Quest 2 (sCMOS) camera equipped with a DoubleHelix add-on for the addition of phase masks
    • 16-bit detector: pixel intensities range from 0 - 65,535

History

Dates Instrument change
02-12-2025 – 02-11-2026 Ran on software NIS Elements 6.10.01
Before 02-12-2025 Ran on software NIS Elements 5

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