Nikon N-STORM - Specifications
General information
Microscope type: TIRF and super resolution
Purpose: STORM- or PALM-based super resolution, TIRF, HiLo, or FRAP
FPbase link: https://www.fpbase.org/microscope/p8tJqsbQSCuVeuQvhbrAM3/
Equipment information
Location: JSCBB C350B
Sample types
| Fixed Cells | Yes |
| Live Cells | Yes |
Software
- NIS Elements 6.10.01
Base
- Nikon Ti2-E
Environmental control
- CO2, Humidity, Temperature
Laser lines
TIRF/STORM
- 405 nm
- 488 nm
- 561 nm
- 647 nm
FRAP
- 405 nm
- 488 nm
- 561 nm
- 640 nm
Filters
| Dichroic Mirror (for Emission) |
Example fluorophores |
|||
| Center (nm) | Width (nm) | Min (nm) | Max (nm) | |
| 450 | 60 | 420 | 480 | DAPI, BFP, CFP |
|
525 |
60 | 495 | 555 | GFP, FITC, Alex 488 |
| 600 | 50 | 575 | 625 | TRITC, Texas Red, Alex 568 |
| 727.5 | 125 | 665 | 790 | Cy5, Alexa 647, Draq5 |
Objectives
| Magnification | NA | Immersion medium |
| 10X | 0.45 | Air |
| 20X | 0.75 | Air |
| 60X TIRF | 1.49 | Oil |
| 100X TIRF | 1.49 | Oil |
Detectors
- Two Andor iXon 897 (EMCCD) cameras equipped with TwinCam for simultaneous imaging of two colors
- 16-bit detector: pixel intensities range from 0- 65,535
- One Hamamatsu Orca Quest 2 (sCMOS) camera equipped with a DoubleHelix add-on for the addition of phase masks
- 16-bit detector: pixel intensities range from 0 - 65,535
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