Nikon N-STORM - Specifications

General information

Microscope type: TIRF and super resolution
Purpose: STORM- or PALM-based super resolution, TIRF, HiLo, or FRAP
FPbase link: https://www.fpbase.org/microscope/p8tJqsbQSCuVeuQvhbrAM3/

Equipment information

Location: JSCBB C350B

Sample types

Fixed Cells	Yes
Live Cells	Yes

Software

• NIS Elements 6.2

Base

• Nikon Ti2

Environmental control

• CO₂, Humidity, Temperature

Laser lines

TIRF/STORM laser box

• 405 nm
• 488 nm
• 561 nm
• 647 nm

FRAP/LUN4 laser box

• 405 nm
• 488 nm
• 561 nm
• 640 nm

Filters

For light coming from FRAP/LUN4 laser box:

Filter (Turret-Hi)	Excitation (nm)	Emission (nm)	Dichroic mirror (nm)	Purpose
LF405-C (405 nm TIRF)	370 – 410	429 – 474	417 – 1200	Calibrating the galvo for FRAP
Continuous STORM	387 – 417 483 – 494 557 – 570 636 – 661	422 – 478 502 – 549 581 – 625 674 – 786	420 – 481 497 – 553 575 – 628 667 – 792	Reflecting any of the lasers to the sample for FRAP
Filter (Turret-Lo)	Excitation (nm)	Emission (nm)	Dichroic mirror (nm)	Purpose
Empty	—	—	—	Allow light from sample to reach the camera

 

For light coming from the TIRF/STORM laser box:

Filter (Turret-Lo)	Dichroic mirror (nm)	Purpose
86000v2 - Sedat Quad	444 – 479 499 – 541 567 – 625 649 – ∞	Reflecting any of the lasers to the sample for TIRF/STORM/laser excitation
Filter (Turret-Hi)	Dichroic mirror (nm)	Purpose
Empty	—	Allow light to reach the sample or the camera

 

For light coming from the Sola II LED box:

Filter (Turret-Lo)	Excitation (nm)	Emission (nm)	Dichroic mirror (nm)	Purpose
DAPI	340 – 380	435 – 485	400 – ∞	Image DAPI, BFP
FITC	465 – 495	515 – 555	505 – ∞	Image EGFP, ATTO 488, FITC
TRITC	528 – 553	577 – 630	565 – ∞	Image RFP, ATTO 565, TRITC
Cy5	590 – 650	663 – 738	660 – ∞	Image iRFP, Alexa 647, Cy5
Filter (Turret-Hi)	Excitation (nm)	Emission (nm)	Dichroic mirror (nm)	Purpose
Empty	—	—	—	Allow light to reach the sample or the camera

Objectives

Magnification	NA	Immersion medium
10X	0.45	Air
20X	0.75	Air
60X TIRF	1.49	Oil
100X TIRF	1.49	Oil

Detectors

• Two Andor iXon 897 (EMCCD) cameras equipped with TwinCam for simultaneous imaging of two colors
  • 16-bit detector: pixel intensities range from 0- 65,535
  • Full sensor: 512 x 512 pixels
  • Field size: (number of pixels in x) x (pixel size in x)
    • 10x objective = 819.2 μm x 819.2 μm
  • Pixel size in x,y: (16.0 μm / objective magnification) x binning²
    • 10x objective at no binning = 1.60 μm x 1.60 μm in x,y
    • 10x objective at 2x2 binning = 6.4 μm x 6.4 μm in x,y
• One Hamamatsu Orca Quest 2 (qCMOS) camera equipped with a DoubleHelix add-on for the addition of phase masks
  
  • 16-bit detector: pixel intensities range from 0 - 65,535
  • Full sensor: 4096 x 2304 pixels
  • Field size: (number of pixels in x) x (pixel size in x)
    • 10x objective = 1884.16 μm x 1059.84 μm
  • Pixel size in x,y: (4.6 μm / objective magnification) x binning²
    • 10x objective at no binning = 0.46 μm x 0.46 μm in x,y
    • 10x objective at 2x2 binning = 1.84 μm x 1.84 μm in x,y

History

Dates	Instrument change
02-12-2025 – 02-11-2026	Ran on software NIS Elements 6.10.01
Before 02-12-2025	Ran on software NIS Elements 5