Nikon N-STORM - Shutdown and clean up

When you are done imaging, remove your sample from the microscope and place it on a kimwipe or other paper towel as it has microscope oil on the bottom.

Shutdown procedure

1. Save your beautiful images and transfer them to your shared drive or flash drive.
2. Remove your sample from the stage and clean up the microscope:
   • Throw any trash in the trash can.
   • Put your stage inserts back on the shelf.
   • If you used a TwinCam filter or a DoubleHelix phase mask, remove them from the light path and put them away.
   • If you used the 100X or 60X oil objectives, clean the oil from the objective following this video.
3. Lower the stage below 1000.
4. Exit Nikon Elements and disconnect from your shared drive.
5. Open the N-STORM reservation calendar.
   • If there is a user within 2 hours of your session, leave the microscope on and proceed to Step 9.
   • If the next user is more than 2 hours after your session, proceed to Step 6.
6. Turn off the Microscope Power Strip (2).
7. Turn off the laser box(es).
   • If you used the FRAP/LUN4 lasers, deselect all laser lines on the box (all the buttons should be grey).
   • Turn the key(s) on the laser box(es) to the "OFF" position.
8. Turn off the switches for the laser boxes and the one labeled "TURN ON!" on the Laser Power Strip (1).
   • Then, turn off the Laser Power Strip itself.
9. Publish an awesome article and recognize us in the acknowledgements, please!

 

Shutdown procedure for environmental chamber

If you used the environmental chamber:

1. Change the Oko Controller settings back to 37 ºC and 5% CO₂.
2. Turn off the Oko Controller (5).
3. Leave the Environmental Power Strip on (4).
4. Close the CO₂ tank valve (top knob only) (6).
5. Detach the humidified gas line from the stage insert.