Nikon AXR - Specifications

General information

Microscope type: Laser scanning confocal
Purpose: High quality 2D and 3D images of fixed and live samples
FPbase link: https://www.fpbase.org/microscope/j6gYWEt6PxGzSA7Qs5UaVX/

Equipment information

Location: JSCBB C350D

CU Tag: UCB 217794, 215396

Sample types

Fixed Cells	Yes
Live Cells	Yes

Software

• NIS Elements 6.02.03

Base

• Nikon Ti2-E

Environmental control

• CO₂, Humidity, Temperature

Laser lines

• 405 nm
• 445 nm
• 488 nm
• 515 nm
• 561 nm
• 594 nm
• 640 nm
• 730 nm

Filters

Emission				Example fluorophores
Center (nm)	Width (nm)	Min (nm)	Max (nm)
446.5	57	418	475	DAPI, BFP, CFP
481.5	35	464	499	CFP
GFP - Cy5 have variable detectors that can be adjusted for any wavelength range.				GFP, FITC, Alexa 488, YFP, TRITC, Alexa 568, mCherry, Alexa 594, Cy5, Alexa 647, Draq5
748.5	203	647	850	Cy5, Alexa 647, Draq5
795	110	740	850	iRFP

Objectives

Magnification	NA	Immersion medium
2X	0.1	Air
10X	0.45	Air
20X	0.75	Air
20X	0.95	Water
60X	1.20	Water
100X	1.45	Oil

Detectors

• Nikon PMT detectors for 405-nm, 445-nm and 730-nm lines
  • 12-bit detectors: pixel intensity values from 0 - 4095
    
• GaAsp variable detectors for 488-nm, 515-nm, 561-nm, 594-nm, and 640-nm lines
  • 12-bit detectors: pixel intensity values from 0 - 4095