Acquiring a z-stack

1. Check the box in the Z tab.
2. Select a mode to define the z-stack: Define the top and bottom, symmetric around a center, or asymmetric around a "center".
3. Choose either Relative or Home as your center point. Relative works with the PFS. Home sets an absolute z value.
4. Define your step settings by altering the step size, the number of steps, and/or the range. The button next to the step size gives the recommended step size based on the objective.
5. Choose which direction you would like to acquire the steps.

Note: Acquiring a z-stack will remove all 'Use PFS' checkboxes in other tabs.