5. Bruker TruLive3D Finding the sample

Make sure you performed the calibration of the system before lowering your sample in the chamber.

  1. Finding your sample

         image viewer.png

LED on.png

NOTE: This is not the focus of the detection objective! It brings the sample in focus for the illumination objective the Blackfly camera is looking through. This step is for identification only.

Find the sample in spinview.png

To acquire the best images from your sample, you need to look at areas that are in the middle of the dish, within the layer of gel that can be imaged. Only the lower 1mm layer of gel at the bottom of the cuvette can be imaged. If your sample is sitting above this, you will not be able to image it. 

The detection objective is fixed, when you move the z stage your are moving the sample dish up and down. The biggest z-stack you  can acquire is around 1mm, that is if you start below the dish and go as far as the working distance of the objective goes. 

The working distance of the objective is what defines your imaging range. However, the images acquired at the limits of this range will not be ideal. If the dish is too high above the detection objective you will be looking at the bottom of the cuvette. If the sample dish is too close from the detection objective, you will be looking far into the gel and this will generate more aberrations.

If you are looking at the edges of the dish, you will also get more aberrations.

The following figures show how to best position the sample and the resulting images with a bead sample.

OptimalSamplePosition.png

SampleTooClose.png

SampleTooFar.png

 



Revision #4
Created 29 February 2024 17:02:23 by Evolene Premillieu
Updated 19 March 2024 16:44:30 by Evolene Premillieu